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Customized Capture NGS

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Target capture sequencing is cost effective because of the significant advantage for projects with large sample sizes and deep sequencing. It is widely applied in clinical sequencing, QTL fine mapping, association studies and result validation for large amount of samples.

MyGenotics GencapTM capture kit provide a comprehensive solution for target capture sequencing, which is customized to the specific capture probes for interesting genomics area (from a few thousand bp to 50 Mb on any portion of the genomic) and hybridized with genomic DNA library. 

MyGenotics GencapTMcapture kit can be well applied to paraffin embedding samples and other clinical samples, and any other species test. We can accept the finished good genomic DNA library, and to return the good capture target area of DNA. This service can be used for exones and introns of genes sequencing(e.g chromosome fragments,etc). We can also provide individual capture kits to meet your needs at the same time. 

We have developed more than 100 Panels for different kinds of diseases:
eurological Disorders 
Metabolic Disease 
Mental Retardation
Hereditary Cardiovascular Disease 
Familial Hypertrophic Cardiomyopathy, Dilated Cardiomyopathy
Hematological System Diseasesquencing
Ophthalmic Diseases
Deafness Disease
Immune system disease
Oncology Disease
Skeleton System Disease
Pathogens detection
HPV integration screening

Project Workflow

Technical advantages
Independent research and development of GencapTM capture technology
2000bp-50M target region capture available
Suitable for all kinds of samples, including FFPE samples
GencapTM capture kit can be individual provided according to your order
The captured target DNA or DNA library can be returned for next experiments 

Sequencing Strategy
150~200 bp insert DNA library

Sequencing Depth
For Mendelian disorder/rare disease: effective sequencing depth above 100×
For tumor sample: effective sequencing depth above 500×

Data Analysis
Advanced analysis including gene fusion and Copy number analysis

Sample Requirements
Input DNA:
For fresh sample: ≥ 1.0 ug (a minimum of 200 ng can be accepted with risk)
For FFPE sample: ≥ 1.5 ug
DNA concentration: ≥ 20 ng/ul
DNA Volume: ≥ 10 ul
OD260/280 = 1.8 - 2.0 without degradation or RNA contamination

Turnaround Time
30 working days for Capture kit design and optimization.
45 working days for sequencing service and data analysis.